Evaluation of the phycocyanobilin and their combinations in a model animal of sclerosis multiple

Abstract

INTRODUCTION: Multiple sclerosis is a chronic inflammatory disease of the central nervous system characterized by generalized areas of focal demyelination. The mainly used animal model is experimental autoimmune encephalomyelitis.

OBJECTIVE: To demonstrate the effects of treatment with phycocyanobilin and their combinations. The PCB is a biliproteina derived from the cyanobacteria Spirulina platensis.

METHODS: EAE was induced in mice C57BL / 6 with MOG35-55 in adjuvant full of Freund/emulsion of Mycobacterium tuberculosis and pertussis toxin. In this model was evaluated the effect of PCB/IFNbeta compared with the active ingredients in an independent way. The daily clinical progression was evaluated, and the concentrations of CCL5, CXCL10, IFN-γ, CXCL2, CCL2, IL-17A, IL-6 and IL-10 in homogenized tissue were determined by ELISA.

RESULTS: EAE mice treated with the PCB/IFN-beta combination showed a significant decrease of the clinical score. On the other hand, PCB/IFN-beta combination treatment induced a significant decrease in the levels of IL-10 and IL-17 in homogenized's brain.

CONCLUSION: The PCB/IFN-beta combination reduces the clinical progression of disease EAE, an effect possibly mediated in the brain by a decrease in the levels of IL-17A and IL-10.